Do you want sustained transgene expression without the risks of introducing exogeneous DNA, such as for developing animal models or pioneering new gene therapy approaches?

SBI's minicircle technology is a great option. If you don’t have time to spend constructing and producing minicircles or the in-house expertise you can make use of SBI Custom Minicircle Services.

Why choose SBI’s CRISPR/Cas9 genome engineering services?

  • Experienced scientific team that routinely produces minicircles for many customers including large pharma partners
  • They clone your cDNA, miRNA, or shRNA into one of their highly-regarded minicircle parental vectors and produce large quantities of minicircles—or only parts of the cloning and production process. They can even customize the parental vector for you.
  • Whether you need 250 µg or 10 mg of minicircles, it can be delivered taking as little as four weeks.
  • Access Minicircle Technology for research use with no licensing restrictions when ordering ready-to-use minicircle DNA through the production service
  • Leverage the minicircle platform for durable gene expression in your most demanding applications

Ordering Minicircle Services is easy

Step 1. Contact the services team for a quote by emailing 
Step 2. Send us your producer plasmid, or have us make the vector for you
Step 3: Receive ready-to-transfect minicircle plasmids as little as four weeks

Produced as small circular DNA fragments after excision from a parental plasmid, the non-viral, episomal minicircle expression cassette is free of any bacterial plasmid DNA sequences that can lead to silencing, and comes with a variety of promoter and reporter combinations. Their small size facilitates more efficient transfection than what’s possible with standard-sized plasmids, and, while minicircles do not replicate with the host cell, expression lasts for 14 days or longer in dividing cells, and can continue for months in non-dividing cells.