CRISPR/Cas9 gRNA and Donor cloning

Do you want sustained transgene expression without the risks of introducing exogeneous DNA, such as for developing animal models or pioneering new gene therapy approaches?
Interested in simplifying your CRISPR/Cas9 genome engineering?

Our supplier SBI can handle design and cloning of your gRNA and homologous recombination donor constructs.
CRISPR/Cas9 technology greatly simplifies genome engineering. However, a successful execution can be surprisingly challenging starting with the careful design and construction of guide RNAs (gRNAs) and homologous recombination (HR) donors. Save yourself time and trouble and have SBI handle this first step

Why choose SBI’s CRISPR/Cas9 genome engineering services?

• Choose either gRNA and HR donor design and construction or their complete Cell Line Engineering Service
• Leverage their well-designed CRISPR/Cas9 products which are used in a number of peer-reviewed papers
• Accomplish more with turn-around times of as little as 1–2 weeks for gRNA cloning, 3–4 weeks for HR donor cloning, and 10–14 weeks for full cell line engineering projects
• Rest easy with your project in the hands of our experienced team that's successfully completed dozens of genome engineering projects
• Enjoy consistent quality, confidentiality, and on-time delivery

Ordering CRISPR/Cas9 Services is easy

Step 1. Contact the services team for a quote by emailing support@sanbio.nl
Step 2. Send us any necessary materials such as plasmids (optional)
Step 3: Receive your constructs in as little as 1–2 weeks for gRNA plasmids, and 3–4 weeks for HR donor plasmids

For gRNAs, SBI can take care of designing and cloning a single gRNA or multiplex gRNAs against a target locus using any SBI SmartNuclease or SmartNickase vector (or customer provided gRNA cloning vector).
For HR donors, SBI can design and clone homology arms into any SBI HR donor plasmid for knock-out, knock-in, tagging, or single nucleotide modification genome engineering projects. When a custom HR donor plasmid is ordered together with custom gRNA design and cloning, the donor vector will not contain the complete gRNA sequences in the homology arms to ensure full compatibility with the gRNA to be used.
Note that SBI’s CRISPR/Cas9 Genome Engineering Services take advantage of our robust and reliable CRISPR/Cas9 products.