Looking for AAV-related products? We can provide you with different AAV-related products such as antibodies, ELISAs, standards and more from our supplier PROGEN?
The adeno-associated virus (AAV) is a small, non-enveloped virus and have a single-stranded DNA (ssDNA) genome of approximately 4.7 kb. Twelve different AAV serotypes are known.
The AAV genome is flanked by two terminal repeats (ITRs) essential for packaging the viral DNA, replication, transcription and site-specific integration. The genome consist of the Rep gene that encodes four rep proteins which are essential for DNA replication, packaging the viral genome and its integration into the host DNA. Also the Cap gene is present in its genome which encodes three viral capsid proteins (VP) that form the virus capsid.
AAV is interested to several gene therapy researcher due to several features such as lack of pathogenicity, the ability to also infect non-dividing cells and has the ability to stably integrate into the host genome at a specific site.
AAV derived vectors possesses very low cytotoxicity and low immunogenicity and rarely integrate into the genome but reside as episomes in the nucleus, lowering the chance of spontaneous gene alteration. AAV enables long-term transgene expression so in theory genes only need to be delivered once. These features makes the AAV-derived vectors a perfect vehicle for the delivery of genetic material to human cells.
We can provide you with many different AAV-related products such as antibodies, ELISAs, standards Dip’n’Check lateral flow tests and Optiprep™ solution for purification of viral particles from our supplier Progen.
Antibodies
The antibodies are great tools to characterize the different stages of AAV infection and to analyze the AAV assembly process. The AAV capsid protein antibodies recognize the linear epitopes of the viral capids, so specifically reactis with denaturated AAV capsid proteins. The AAV particle antibodies recognize a conformational epitope of the assembled capsids, so specifically react with intact AAV particles. Also AAV replicase antibodies are available for your research.
| AAV capsid protein antibodies | AAV particle antibodies | AAV replicase antibodies |
| Anti-AAV VP1
(Cat No. 61056) |
AAV1 | AAV2 replicase reacting with Rep78 and rep52 (Cat No. 61073) |
| Anti-AAV VP1/VP2
(Cat No. 61057) |
AAV2 (Cat No. 61055, 615298, 65155, 610298, 615298 | AAV2 replicase with low affinity with Rep78, Rep68, rep52 and Rep40 (Cat No. 65172) |
| Anti-AAV VP1/VP2/VP3 | AAV3 recognized by A20 AAV2 and ADK8 AAV8 | AAV2 replicase reacting with Rep78, Rep68, Rep52 and rep40 (Cat No. 61071) |
| AAV4 (Cat No. 610147, 615147)
|
AAV2 replicase reacting with Rep78, Rep68, Rep52 and rep40 (Cat No. 61069) | |
| AAV5 (Cat No. 610137, 610148, 615148, 610149) | ||
| AAV6 (Cat No. 610159, 615159) | ||
| AAV7 recognized by ADK8 AAV8 | ||
| AAV8 (Cat No. 610160, 615160, 651160, 690161) | ||
| AAV9 (Cat No. 690161, 690162, 610178) | ||
| AAVrh10 recognized by ADK8 AAV8) | ||
| AAV12 recognized by ADK1a AAV1 | ||
| AAVrh74 recognized by ADK8 AAV8 and ADK8/9 AAV8 & AAV9 | ||
| Anc80 recognized by A20 AAV2 and ADK8 AAV8 | ||
| AAVDJ recognized by ADK8/9 AAV8 |
ELISAs
You can rely on PROGEN’s AAV ELISAs to consistently deliver dependable capsid titer determination. For most commonly used AAV serotypes both AAV titration ELISAs and Xpress ELISAs are available. The assay time of the Xpress ELISAs is reduced more than 50% compared to standard kits, so results in less than two hours. Both PROGEN’s AAV ELISA and AAV ELISA Xpress kits have a reference standard status in gene therapy centers worldwide.
| AAV type | Titration ELISA Cat No. | Xpress ELISA Cat No. |
| AAV1 | PRAAV1 | |
| AAV2 | PRATV, PRAAV2R | PRAAV2XP |
| AAV3 | PRAAV3R | |
| AAV5 | PRAAV5 | PRAAV5XP |
| AAV6 | PRAAV6 | PRAAV6XP |
| AAV8 | PRAAV8 | PRAAV8XP |
| AAV9 | PRAAV9 | PRAAV9XP |
| AAVrh10 | PRAAV10 |
Standards
PROGEN’s fully characterized AAV standards are prefect to monitor the validity of your results and to ensure comparability of your assays.
AAV Empty Capids (AAV1, AAV2, AAV5, AAV6, AAV8 and AAV9) are offered in liquid form with high titers of more than 5.0E+12 viral particles/ml and can be used as reference material in a variety of applications including ELISA, Western blot and dot blot.
AAV ELISA controls can be used as positive controls with the corresponding AAV ELISAs.
| AAV type | Empty capsids Cat No. | ELISA controls Cat No. |
| AAV1 | 66V010 | PRAAV1-C |
| AAV2 | 66V020 | PRAAV2-C |
| AAV3 | PRAAV3-C | |
| AAV5 | 66V050 | PRAAV5-C |
| AAV6 | 66V060 | PRAAV6-C |
| AAV8 | 66V080 | PRAAV8-C |
| AAV9 | 66V090 | PRAAV9-C |
| AAVrh10 | PRAAV10-C | |
| AAVrh74 | PRAAV74-C |
AAV VP Capsid protein standards can be used to monitor VP1, VP2 and VP3 protein concentrations and their molar ratios to ensure initial optimization and consistent quality of your final AAV gene therapy products
| VP recombinant protein standards description | VP protein standards Cat No. | VP recombinant protein standards description | VP protein standards Cat No. |
| AAV2 VP1 + VP2 + VP3 | 72001 | AAV8 VP1 + VP2 + VP3 | 72008 |
| AAV2 VP1 | 640823 | AAV8 VP1 | 640839 |
| AAV2 VP2 | 640824 | AAV8 VP2 | 640840 |
| AAV2 VP3 | 640825 | AAV8 VP3 | 640841 |
| AAV5 VP1 + VP2 + VP3 | 72005 | AAV9 VP1 + VP2 + VP3 | 72009 |
| AAV5 VP1 | 640833 | AAV9 VP1 | 640842 |
| AAV5 VP2 | 640834 | AAV9 VP2 | 640843 |
| AAV5 VP3 | 640835 | AAV9 VP3 | 640844 |
| AAV6 VP1 + VP2 + VP3 | 72006 | ||
| AAV6 VP1 | 640836 | ||
| AAV6 VP2 | 640837 | ||
| AAV6 VP3 | 640838 |
Dip'n'Check lateral flow tests
Testing individual and crude AAV samples quick during development and manufacturing is often difficult, because most accurate analytical AAV tools are not convenient for single sample measurement and/or require highly purified samples. PROGEN’s AAV Dip’n’Check lateral flow test are quick and easy to check the AAV capsid purification process and ideal for analytical pre-testing.
Detection of AAV1 and AAV6 capsids: Cat No. PR5216
Detection of AAV2 and AAV3 capsids: Cat No. PR5223
Detection of AAV8 capsids: Cat No. PR5208
Detection of AAV9 capsids: Cat No. PR5209
Optiprep™ solution
Optiprep™ Cat.no 1893 is the ideal solution for virus purification such as AAV, because the ratio of infectious particles to total particles is 100 times higher using Optiprep™ gradients compared to CsCl gradients.
Using OptiPrepTM for AAV purification saves time, because no need to remove iodixanol for most downstream processes, it causes little or no damage to viral particles, results in a then times greater recovery rate than CsCl gradient alternatives and results in a higher infectively titer with no toxic effects.