For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. A20 specifically reacts with intact adeno-associated virus 2 and 3 particles , empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins. The antibody cannot be used for immunoblotting.Epitope mapping experiments (Wobus, C. E. et al., 2000) identified four immunoreactive (discontinous) regions. The major reaction was attributed to sequence aa 369 to aa378 of AAV2 capsids. The anti-body is also useful for neutralizing experiments (Moskalenko, M. et al., 2000).
Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).Moskalenko, M. et al. Epitope Mapping of Human Anti-Adeno-Associated Virus Type 2 Neutralizing Antibodies: Implications for Gene Therapy and Virus Structure. Jounal Virol. 74, 1761–1766 (2000).
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