IBA Lifescience

Sanbio, authorized distributor of the Benelux!

IBA Lifesciences GmbH, located in Göttingen, Germany, is a biotechnology company providing products for life science research in academia and industry. Leveraging their exclusive Strep-tag® technology, they engineer cutting-edge tools for cell and protein isolation.

High performance research tools for cell and protein isolation

View all IBA Lifescience products

Product portfolio

Affinity Chromatography
Antibody Development
Antigen-specific Cell Isolation
Biotin Blocking
Cell Isolation
Cloning
ELISA
Flow Cytometry

High-Throughput Purification
Protein Purification
Protein-Protein Interaction
Pull-Down
SPR & BLI
Staining & Detection
Transfection
Western Blot

Strep-tag® technology

Cutting-edge tools for high-performance research in cell and protein isolation are enabled by the Strep-tag® technology, leveraging the robust interaction between biotin and streptavidin.

This technology harnesses the specificity and controllability of the interaction between the synthetic Strep-tag®II peptide and engineered streptavidins like Strep-Tactin®, renowned for its stability. By occupying the biotin binding site, Strep-tag®II forms a reversible interaction with engineered streptavidins, Strep-Tactin® and Strep-Tactin®XT easily disrupted by excess biotin.

The Strep-tag®II, comprising eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys), and its variant, Twin-Strep-tag® is composed of 28 amino acids inluding two of these motifs in tandem connected by a linker. Both exhibit varying affinities for Strep-Tactin® derivatives. Strep-tag®II shows significantly higher affinity for Strep-Tactin® compared to streptavidin, with an affinity nearly 100 times stronger. The development of Strep-Tactin®XT further enhances affinity, which shares nanomolar affinity with Strep-tag®II and picomolar affinity for Twin-Strep-tag®.

Due to variations in binding strength among different tag-ligand combinations, the Strep-tag® system has emerged as a versatile tool for isolating proteins and cells.

The Strep-tag® fused to recombinant proteins facilitates efficient one-step purification when immobilized on Strep-Tactin®.
When attached to antibody-derived Fab fragments or nanobodies, the Twin-Strep-tag® interacts with multimerized Strep-Tactin®, enabling the capture and release of target cells based on their surface markers or antigen specificity.
Strep-Tactin® (or Strep-Tactin®XT) can be conjugated to microplates, fluorophores, or chips, wich makes this tool suitable for a wide range of analytical applications following the isolation of target material, such as detection, immobilization, and interaction studies.