CytoTrack Cell Proliferation Assays efficiently stain live cells to obtain excellent resolution of each cell division. These Dyes enable the measurement of cell proliferation in live cells using flow cytometry. As the labeled cells divide, the concentration of the dye is halved, and the proliferation is measured, based upon the reduced levels of fluorescence in subsequent generations. Using proprietary chemistry, the dye reacts with intracellular proteins and is retained in the cell with minimal efflux, allowing the CytoTrack Cell Proliferation Assays to track up to ten cell generations in dividing cells.

Key benefits:

  • Nontoxic — minimal cellular toxicity
  • Detection of intracellular targets — compatible with standard formaldehyde-containing fixatives and saponin-based permeabilization buffers
  • Suitability for multicolor cell analysis — a range of excitations and emissions available for compatibility with fluorescent proteins or fluorophore-labeled antibodies
  • No washing required — use the cell proliferation assay directly with your cell culture media
  • Suitability for long term tracking of labeled cells — resolve up to 10 cell divisions compared with approximately 8 cell divisions with CFDA-SE

Proliferation Dyes

Description Excitation Max, nm Emission Max, nm Optimal Excitation Laser Catalog Number
CytoTrack blue

403

454

405

1351202

CytoTrack green

511

525

488

1351203

CytoTrack red

628

643

640

1351205

CFDA-SE

494

521

488

1351201