What are Recombinant Prokaryotic Lectins (RPLs)?

  • RPLs are glycoselective bioaffinity compounds, they recognize and bind to specific glycan structures displayed on glycoproteins and other glycosylated biomolecules.
  • Recombinant Prokaryotic Lectins can enable simple, fast, and efficient detection, analysis, separation, and purification of intact glycosylated biomolecules.
  • Unlike with traditional plant & eukaryotic lectins, RPLs (recombinant prokaryotic lectins) production easily delivers high-quality products with consistent performance and enhanced glycan specificity and affinity. This property enables higher yields of these molecules and makes them suitable for applications in larger-scale separation and purification methods.

Recombinant Prokaryotic Lectins of GlycoSelect Ltd.

GlycoSeLect specialises in the development of proteins based on recombinant prokaryotic lectins RPLs. Their RPL products advance efficient detection, analysis and selective isolation of glycosylated pharmaceutical products. GlycoSeLect’s family of recombinant prokaryotic lectin products (RPLs) are associated with market-leading properties, including high affinity for glycan targets, high sensitivity of detection, high stability and robustness in process development.

Available Recombinant Prokaryotic Lectins

GlycoSeLect has developed a range of RPL products exhibiting specificity and high affinity for a range of glycan epitopes displaying terminal galactose and mannose (including high mannose structures).

All recombinant prokaryotic lectins display a poly-histidine tag enabling simple detection using standard conjugated anti-his detection antibodies negating a requirement for in vitro labeling (e.g. biotinylation).

Products are available in a range of pack sizes and can be supplied in large quantities (grams) if required.

RPL-αGal RPL-GAL1 RPL-GAL2
RPL-GAL3 RPL-GAL4 RPL-αMan
RPL-Man2 RPL-Sia1 RPL-Sia2
RPL-Sia3 RPL-Fuc1

Applications

Biopharmaceutical

The recombinant prokaryotic lectins of GlycoSelect can be easily integrated into a wide range of analytical platforms and have multiple applications, from product development to production. The diversity of solid support matrices that RPLs can be immobilized on and the easy scalability of their production, allows huge flexibility in glycoselective bioaffinity-based purification, even for large scale use.

Diagnostic & Research

Recombinant prokaryotic lectins provide a great tool for identification of novel glycan-based biomarkers and present an opportunity for new diagnostic approaches. They can also be used for the detection and analysis of glycans in situ, for the purposes of cell identification, and the classification of microorganisms or other infectious agents.

Food Industry

Recombinant prokaryotic lectins can be used to extract valuable glycoproteins from food industry raw material streams.

Cosmetics

Glycans play an important role in maintaining the structure and health of the skin. RPLs represent useful tools for studying the glycobiology of the skin. They can be used as biochemical reagents in a wide range of assay formats or integrated into analytical platforms to enable the detection and analysis of glycosylated biomolecules.

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