Stable Cell Line Development Service by OriGene
Generating custom cell lines is a tedious and complex process. OriGene offers a custom stable cell line development service that can be designed for knockdown, knock-in, or knockout of virtually any protein of interest (constitutive and inducible options available). Take advantage of their expertise in gene expression and highly effective gene delivery technologies to produce high-quality cell lines.
What they offer
- Over Expression (ORF)
- Knockdown (shRNA)
- Knock-in (CRISPR)
- Knockout (CRISPR)
Service Highlights
- Fast turnaround time: 2-3 months
- Choose from OriGene’s extensive ORF clone collection or provide your own expression cassette
- Rigorous and customizable QC validation process
- Professional, Global Technical Support
Cell Line Development Workflow
Inducible Stable Cell Line Development
Inducible expression is a valuable tool for functional analysis of assay systems with toxic targets. OriGene's All-in-one Tet-On system is a new and improved version of the original Tet-On systems, designed to significantly stimulate expression of the downstream gene of interest (GOI).
Custom Overexpression stable cell lines by System BioSciences
Reliable, high-quality custom stable cell line generation for overexpression of your protein- or regulatory RNA-of-interest
Leverage SBI’s viral and non-viral vectors in robust overexpression cell lines
Whether you want constitutive or inducible overexpression of a protein or regulatory RNA, SBI’s services team can create the construct and the cell line. They have access to a range of technologies—including stably integrating viral and non-viral vectors—and an expert team experienced at working with a variety of cell lines, including adherent and suspension cells. The result is hundreds of successfully constructed stable cell lines for highly satisfied customers.
Why choose SBI’s Stable Cell Line Services?
- Save time by having SBI build your custom cell lines
- Leverage our highly-regarded gene expression and delivery technologies
- Accomplish more with our fast turnaround times and end-to-end services
- Turn to the company that other companies turn to—we are a trusted stable cell line manufacturer for many large pharma/biotech companies
- Enjoy consistent quality, confidentiality, and on-time delivery with all projects completed on-site in our Palo Alto, CA, facility
Available gene expression and delivery technologies
Viral Systems
- Lentivectors—high titer and reliable
- Random integration
- Limited insert size
- High copy number
- Lentivirus Packaging—reliable gene delivery
- High and ultra-high titers
- Efficient transduction
- Optimized reagents
Non-viral Systems
- PhiC31 Integrase System—one-step single-copy integration
- Site-specific integration
- Unlimited insert size
- Single copy number
- PinPoint Targeted Integration System—excellent for creating isogenic cell lines
- Site-specific integration
- Unlimited insert size
- Single copy number
