Rabbit anti Human anticoagulation Factor IX

The defined antibody reactivity is restricted to Factor IX as tested at the level of sensitivity of immuno-precipitation techniques. A single precipitin line is obtained in bidimensional electrophoresis, immunoelectrophoresis and double dial immunodiffusion (Ouchterlony) which shows a reaction of full identity with the purified immunogen. No precipitation is obtained with any other plasma protein and with haemophilia B plasma. n precipitating techniques as electroimmunodiffusion, immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony). To prepare an adsorbent for immunoaffinity purification of FIX. If used in more sensitive test procedures or as catching or detection antibody in solid phase immunoassays specificity controls should always be include. Plasma samples and all assay components must contain EDTA to stabilize the proteins. Normal adult plasma contains an average of 1 mg FIX per 100 ml. In a normal population this varies between 80% and 120% of the average. Newborn infants have a level of about 75% of the average adult level. Absence of FIX in plasma causes a bleeding diathesis very similar to haemophilia A in clinical manifestations and from the point of view of heredity. It is known as haemophilia B or Christmas disease. Different types have been described. The most common type demonstrates reduced FIX coagulant activity without measurable FIX relates antigen. Other types of reduced FIX antigen and coagulation activity, or reduced coagulant activity with normal antigen levels. Heterozygote carriers can be detected. Acquired depression of plasma FIX levels are seen in vitamin K deficiencies, in liver disease, or following therapy with vitamin K antagonists (oral coagulants).