Anti-monomeric Kusabira-Orange 2 mAb

CoralHue® Kusabira-Orange (KO) has been cloned from the stony coral, whose Japanese name is “Kusabira-ishi”. Wild-type CoralHue® KO forms a brightly fluorescent dimer. CoralHue® KO has been carefully engineered to form a monomer, CoralHue® monomeric Kusabira Orange 1 (mKO1) that maintains the brilliance and pH stability of the parent protein. CoralHue® mKO2 is the mutant of mKO1 and has a feature of the rapid maturation. It absorbs light maximally at 551 nm and emits orange light at 565 nm. CoralHue® mKO2 can be used to label proteins or subcellular structures, or for reporter assay. M168-3 is available for immunostaining of “Fucci-G1 Orange” (Fucci; Fluorescent Ubiquitination-based Cell Cycle Indicator). Fucci-G1 Orange encodes CoralHue® monomeric Kusabira-Orange2 (mKO2) fused to a part of human Cdt1 (hCdt1: Cdc10 dependent transcript 1). It is possible to use M168-3 for Fucci transgenic strain, B6.Cg-Tg(Fucci)596Bsi mice which express Fucci-G1 Orange.