Protein A Magnetic Beads IP/Co-IP Kit

TrueBlot® Protein A Magnetic Beads IP/Co-IP Kit allows highly effective and efficient immunoprecipitation (IP) and co-immunoprecipitation (co-IP) of an antigen using smaller amounts of antibody (typically 1-10 µg). The kit is designed for immunoprecipitation of proteins, protein complexes, protein-nucleic acid complexes and other antigens. The specific antibody is added to the sample to form an immune complex. The immune complex is then bound to Protein A Magnetic beads. The complex is washed to remove unbound material and the bound immune complex is then eluted from the beads using low-pH elution buffer. Alternatively, the sample buffer is used for dissociation using denaturing conditions or for down-stream process for SDS-PAGE analysis. The kit includes Protein A magnetic beads for high capture efficiencies and optimized buffers for high yield of target antigen. Bead mean diameter is ~0.5 ?m, bead concentration is 5 mg/mL. The kit contains magnetic beads, Binding/Wash buffer, Elution buffer, and Neutralization buffer sufficient for 20 samples or tests. (See protocol for details). The beads are removed from the solution using a magnetic separator.