Human Anti-Cyclophilin A

Human anti cyclophilin A antibody, clone AbD00794 recognizes cyclophilin A, also known as peptidyl-prolyl cis/trans isomerase A, PPIA or rotamase A. Cyclophilin A is a ~18 kDa, ubiquitously distributed intracellular protein that can be secreted by cells in response to inflammatory stimuli (Jin et al. 2000).Cyclophilin A is a member of the immunophilin class of proteins that all possess peptidyl-prolyl cis/trans isomerase activity and, therefore, are believed to be involved in protein folding and/or intracellular protein transport (Fischer et al. 1989). This protein can interact with several HIV proteins including p55 gag, Vpr, and capsid protein. It has been shown to be necessary for the formation of infectious HIV virions (Franke et al. 1994). Cyclophilin is a specific high-affinity binding protein for the immunosuppressant agent cyclosporin A (Handschumacher et al. 1984). Because of its dramatic effects on decreasing morbidity and increasing survival rates in human transplants, the molecular mechanism of immunosuppression by cyclosporin A has been a matter of much interest (Durette et al. 1988). Multiple PPIA pseudogenes that map to different chromosomes have been reported. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed (Chatellard-Gruaz D et al. 1994).Human anti cyclophilin antibody, clone AbD00794 has been used successfully for the detection of cyclophilin A in HeLa cell lysates by Western blotting.