Goat anti Canavalia Concanavalin A, conjugated with Horseradish peroxidase

Tested in immunoelectrophoresis, using different antigen/antibody concentration ratio’s the antiserum gives one characteristic precipitin line. In enzyme-immunocytochemical and immunohistochemical staining of concanavalin A in appropriately prepared substrates. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. The entire test system must be free of sodium azide, since it inhibits the enzyme activity.