Anti-GFP

Originally derived from the jellyfish Aequorea Victoria, Green fluorescent protein (GFP) has become an invaluable tag and reporter protein in molecular and cellular biology. GFP has a molecular weight of 26.9 kDa and when excited with blue light at 395nm it will fluoresce green with an emission peak at 509nm (1). The GFP structure is made up of a fluorophore containing alpha helix that is enveloped by a beta barrel that protects the fluorophore from localized quenching when excited (1).