CPB (carboxypeptidase B), Active

Carboxypeptidase B (CPB) is a metal-containing pancreatic endopeptidase that catalyzes the successive release of basic carboxy-terminal residues, such as Arg and Lys. CPB also targets other amino acids, albeit at a slower rate. These secondary target residues include valine, leucine, isoleucine, asparagine, glycine, and glutamine. CPB depends on Zn2+ to promote enzymatic activity. Conversely, the enzyme is inhibited by some heavy metals, including Cu2+, Hg2+; metal chelating agents such as EDTA and competing Arg and Lys residues. CPB functions between pH 5-12, with an optimal pH of 8. Enzyme activity also increases with rises in temperature. CPB is used in the production of recombinant human insulin and monoclonal antibody/IgG1 (mAb) processing.