pFucci-S/G2/M Green(N+C) (Cloning vector)

AM-V9034 pFucci-S/G2/M Green (N+C) is a Cloning vector encoding CoralHue® humanized-codon monomeric Azami-Green1 (hmAG1) fused to a part of human Geminin (hGeminin). pFucci-S/G2/M Green (N+C) can trace the silhouette of individual cells in S/G2/M phases with fluorescence. “Fucci” stands for Fluorescent Ubiquitination-based Cell Cycle Indicator. Geminin is an inhibitor of the DNA replication licensing factor. It accumulates during the S, G2, and M phases, but is degraded during G1 phase by ubiquitin-mediated proteolysis. A part of hGeminin (1-60) is also degradable in a cell cycle dependent manner. CoralHue® hmAG1 sequence is codon-optimized for higher expression in mammalian cells. CoralHue® monomeric AG1 (mAG1) has been generated from tetrameric CoralHue® Azami-Green (AG).