Goat TrueBlot® Western Blot Kit

The Goat TrueBlot® Western Blot Kit contains the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blot of samples using TrueBlot monoclonal secondary antibody in conjunction with your own primary IP antibody and primary (Goat IgG) Western blot antibody (see application note for kit components). Goat IgG TrueBlot® is the unique horseradish peroxidase conjugated Anti-Goat IgG monoclonal secondary antibody which enables detection of immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your conventional HRP Anti-Goat IgG immunoblotting secondary antibody. It is easy to generate publication?quality IP/WB data with Goat IgG TrueBlot. Goat IgG TrueBlot® is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of goat IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Goat IgG TrueBlot® with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/immunoblotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Goat IgG TrueBlot® may also be used for detection in immunoblotting assays that do not employ immunoprecipitation.