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B-Hydroxybutyrate (Ketone Body) Fluorometric Assay Kit
700740-96
96 wells
€420
Product description
Cayman’s ?-HB (Ketone Body) Fluorometric Assay Kit provides a simple, reproducible, and sensitive tool for assaying ?-HB from plasma, serum, urine, tissue homogenates, and cell lysates. The method for ?-HB determination is based upon the oxidation of D-3-Hydroxybutyrate to acetoacetate by the enzyme 3-hydroxybutyrate dehydrogenase.16 Concomitant with this oxidation, the cofactor NAD+ is reduced to NADH. NADH reacts with the fluorometric developer to yield a highly fluorescent product which can be analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm. The fluorescence is directly proportional to the ?-HB concentration.
Specifications
Supplier
Cayman Chemical
Shipping & storage
Shipping condition
Dry Ice
Storage temperature
-20°C
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B-Hydroxybutyrate (Ketone Body) Fluorometric Assay Kit
700740-96
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B-Hydroxybutyrate (Ketone Body) Fluorometric Assay Kit
700740-96
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