MR1 Immunoaffinity Sorbent

Major histocompatibility complex (MHC) class I-related gene protein (MR1) is a non-polymorphic MHC class Ib antigen-presenting cell surface molecule that is required for T cell receptor-mediated activation of mucosal-associated invariant T (MAIT) cells.{60473,60472} It is composed of ?1 and ?2 domains, which form an antigen-binding pocket, and an ?3 domain that interacts with ?2-microglobulin.{60472} MR1 mRNA is ubiquitously expressed and, following translation, MR1 protein is localized to the endoplasmic reticulum in a partially folded state. Upon binding of a riboflavin-derived microbial antigen, MR1 undergoes a conformational change and translocates to the cell surface where it induces MAIT cell activation via an interaction with the MAIT cell T cell receptor and activates various immunomodulatory effects, including cytokine release, initiation of adaptive immune responses, and promotion of tissue repair.{60474} Cayman's MR1 Immunoaffinity Sorbent is designed for immunoprecipitation (IP) of MR1 protein from biological samples. This is an effective way to concentrate MR1 protein for subsequent detection by a different MR1 antibody by immunoprecipitation (IP) (LC/MS certified) and Western blot (WB). The MR1 affinity sorbent consists of Cayman's MR1 Monoclonal Antibody (Clone 2H7) (Item No. 34257) coupled to CNBr-activated Sepharose 4B.