EpCAM Rabbit Monoclonal Antibody

Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein.{64848} It is composed of an extracellular domain (EpEX) that contains an N-domain, a thyroglobulin type-IA domain, and a C-domain, a transmembrane domain, and an intracellular domain (EpIC). EpEX undergoes proteolytic cleavage by disintegrin and metalloproteinase domain-containing protein 17 (ADAM17/TACE) to release soluble EpEX, and ?-secretase cleaves EpCAM at two sites to release a soluble extracellular amyloid-?-like fragment and EpIC. EpCAM is ubiquitously expressed in epithelial tissues but is also found in stem and progenitor cells and tight junctions.{64849,64850} It is involved in cell adhesion, transport, polarity, proliferation, and motility and signals through novel PKC (nPKC), Wnt, and embryonic RAS (ERAS)/Akt pathways.{64849} Knockdown of EPCAM decreases proliferation, migration, and invasion of breast cancer cells in vitro.{14599} Epcam-/- mice develop congenital tufting enteropathy (CTE), a disease characterized by hemorrhagic diarrhea, colon crypt hyperplasia, intestinal tufts, and villous atrophy.{64851} Increased protein levels of EpCAM are frequently found in tumors from patients with diverse cancer types.{14601} Cayman’s EpCAM Rabbit Monoclonal Antibody (Clone 28) can be used for ELISA, ELISA (detection), flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry paraffin (IHC-P), immunoprecipitation (IP), and Western blot (WB) applications. The antibody recognizes EpCAM at 34 kDa from human samples. However, due to glycosylation the antibody may recognize EpCAM at approximately 38-40 kDa.