Citrullinated Vimentin Monoclonal Antibody - Biotinylated (Clone 12G11)

Vimentin is a cytoskeleton intermediate filament protein present in cells of mesenchymal origin, including leukocytes, endothelial cells, and smooth muscle cells. It is subject to citrullination by peptidyl arginine deiminase 2 (PAD2; Item No. 10785) under high calcium concentrations, which can occur during macrophage apoptosis.{31884} Citrullinated vimentin has been shown to have a role in the production of anti-citrullinated protein antibodies (ACPAs).{31883,31884} Serum levels of immune complexes targeting citrullinated vimentin are increased in patients with rheumatoid arthritis and positive for ACPAs.{66176} Serum and plasma levels of citrullinated vimentin are also increased in patients with lung cancer or idiopathic pulmonary fibrosis, respectively.{35094,35741} Cayman's Citrullinated Vimentin Monoclonal Antibody - Biotinylated (Clone 12G11) can be used for ELISA, immunoprecipitation (IP), and Western blot (WB) applications. The antibody recognizes citrullinated vimentin at approximately 54 kDa from human samples.