Ubiquitin Remnant (K-?-GG) Immunoaffinity Sorbent

Ubiquitin remnant (K-?-GG) is a diglycyl-lysine motif found in proteins subject to ubiquitination, a process for tagging proteins for proteasomal degradation that is disrupted in certain diseases.{65646,65647} During ubiquitination, ubiquitin is covalently bound to the ?-NH2 in lysine residues of the target protein via the C-terminal carboxyl group of ubiquitin, which is preceded by arginine-glycine-glycine (-RGG) residues.{65648} Ubiquitinated proteins can be detected, and enriched for, via antibodies toward the K-?-GG motif resulting from this covalent bond.{65646,65649} Cayman's Ubiquitin Remnant (K-?-GG) Immunoaffinity Sorbent is designed for immunoprecipitation (IP) of ubiquitin remnant (K-?-GG) motif-containing proteins from biological samples. This is an effective way to concentrate ubiquitin remnant (K-?-GG) motif-containing proteins for subsequent detection and analysis by LC-MS/MS. The ubiquitin remnant (K-?-GG) affinity sorbent consists of Cayman's Diglycyl-Lysine Monoclonal Antibody (Clone GX41) (Item No. 21096) coupled to CNBr-activated Sepharose 4B.