12-Lipoxygenase (leukocyte-type; mouse) Polyclonal Antibody

12-Lipoxygenase (12-LO) is one of several LOs that catalyzes the peroxidation of polyunsaturated fatty acids in a position-specific manner.{59716} There are four 12-LO subtypes, encoded by distinct genes, that can be categorized according to their tissue expression in mice.{59714} Leukocyte-type 12-LO is encoded by Alox15 in mice and shares 74% sequence identity with human ALOX15 but, in contrast to its human ortholog, encodes a 12-lipoxygenating ALOX15 isoform.{54602,59715,59713} Leukocyte-type 12-LO exists as a single polypeptide chain composed of an N-terminal regulatory domain and a C-terminal catalytic domain.{59716} It catalyzes the oxidation of arachidonic acid (Item Nos. 90010 | 90010.1 | 10006607) to 12(S)-HpETE (Item No. 44570) and, to a lesser extent, 15(S)-HpETE (Item No. 44720), which are further reduced to 12(S)-HETE (Item No. 34570) and 15(S)-HETE (Item No. 34720), respectively.{59716,33744,3606} Other substrates of leukocyte-type 12-LO include eicosapentaenoic acid, docosahexaenoic acid, linoleic acid, and y-linolenic acid, which are oxygenated at various positions in a substrate-dependent manner.{59717} Leukocyte-type 12-LO is constitutively expressed at high levels in resident peritoneal macrophages, and its activity is increased by the Th2 cytokines IL-4 and IL-13 in vitro.{59713} Alox15-/- mice are protected from increases in systolic blood pressure in a model of hypertension induced by L-NAME (Item No. 80210).{59718} Cayman's 12-Lipoxygenase (leukocyte-type; mouse) Polyclonal Antibody can be used for ELISA and Western blot (WB) applications. The antibody recognizes 12-LO (leukocyte-type) at ~73 kDa from porcine and mouse samples.