Histone H4 is one of four core histone proteins that are involved in the organization of DNA into chromatin.{18136} Histones are globular proteins with unstructured N-terminal tails and are subject to a variety of post-translational modifications, such as methylation, acetylation, phosphorylation, and citrullination, that can influence chromatin structure and regulate gene transcription.{18136,57056} Acetylation of histone H4 at lysine 20 (H4K20Ac) is associated with gene repression.{59477} It is enriched near the transcription start sites of minimally expressed genes and, to a lesser extent, in the gene body regions of highly expressed genes in HeLa-S3 cells. H4K20Ac can be recognized and bound by the bromodomain of the histone acetyltransferase CREB-binding protein (CBP), as well as by the second bromodomain of bromodomain-containing protein 4 (BRD4).{59478} Cayman’s Histone H4K20Ac Monoclonal Antibody can be used for chromatin immunoprecipitation (ChIP), ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.