Histone H3K9Me2 Monoclonal Antibody (Clone RM151)

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.{57056} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.{57056,53978} Dimethylation of histone H3 at lysine 9 (H3K9Me2) is associated with transcriptional repression.{48220} Heterochromatin protein 1-? (HP1-?), HP1-?, and HP1-? selectively bind histone H3 N-terminal peptides containing dimethylated lysine 9 over unmodified lysine 9.{20846} High nuclear levels of H3K9Me2 in tumor tissue are associated with decreased disease-specific and disease-free survival in patients with oral and oropharyngeal squamous cell carcinoma.{49115} Cayman’s Histone H3K9Me2 Monoclonal Antibody (Clone RM151) can be used for chromatin immunoprecipitation (ChIP), ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.