Histone H3K36Me3 Monoclonal Antibody

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.{57056} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.{57056,53978} Trimethylation of H3K36 is increased in the G1 and early S phases of the cell cycle where it binds to and recruits the mismatch recognition protein MutS? in preparation for DNA replication and mismatch repair.{46225} H3K36 trimethylation is found in greater amounts on exons compared with introns in C. elegans, mouse, and human genome-wide maps of histone H3 tail methylations.{46226} The loss of H3K36Me3 is correlated with poor prognosis in multiple cancer types while high levels increase expression of tumor suppressor genes.{56220} H3K36Me3 is negatively correlated with esophageal squamous cell carcinoma (ESCC) metastasis and positively correlated with increased survival in patients with ESCC. Cayman’s Histone H3K36Me3 Monoclonal Antibody can be used for chromatin immunoprecipitation (ChIP), ELISA, multiplex-based assay, and Western blot (WB) applications.