APOA5 Recombinant Protein

Source : Escherichia Coli. APOA5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 366 amino acids (24-366 a.a.) & having a molecular mass of 41.3kDa.APOA5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. ApoA5 is an important regulator of serum triglyceride concentrations. An ApoA5 mouse nock-out model produced an approximately four fold increase in serum triglycerides, whereas a knock-in model human ApoA5 produced 50-70% lower concentrations of mouse serum triglycerides. Furthermore, peroxisome proliferators-activated receptoragonists, which are used clinically to lower serum triglyceride concentrations, cause increased ApoA5 mRNA expression. There is very little known about ApoA5 protein in human serum. Lately, it was established that ApoA5 is present in the human serum & it can be detected by polyclonal antibodies against both the HN2 & COOH termini, but at much lower concentration than other apolipoproteins.