Optineurin (C-Term) Monoclonal Antibody (Clone 8E12)

Optineurin (OPTN) is an adapter protein with roles in autophagy, vesicular trafficking, and inflammatory responses to viral and bacterial pathogens.{47745} OPTN binds to ubiquitinated cargo via its ubiquitin-binding domain and targets them for autophagic degradation by recruiting LC3-expressing phagophore membranes via its LC3-interacting region. It is phosphorylated by TANK binding kinase 1 (TBK1), which increases the binding affinity of OPTN for polyubiquitinated mitochondria, promotes TBK1 activation and recruitment to the mitochondria, and permits the degradation of damaged mitochondria via PINK1/parkin-dependent mitophagy.{47746} OPTN regulates transferrin receptor trafficking and recycling by mediating the association between the membrane trafficking protein Rab8 and its activator TBC1D17.{47747} Mutation of the OPTN ubiquitin binding domain reduces LPS- and poly(I:C)-induced increases in IFN-? levels in bone marrow-derived macrophages (BMDMs).{47748} OPTN knockout increases mortality and reduces plasma levels of TNF-? and IL-6 in a mouse model of E. coli-induced peritonitis.{47749} Mutations in OPTN are associated with primary open angle glaucoma, amyotrophic lateral sclerosis (ALS), Paget's disease, and Alzheimer's disease.{47745,47750} Cayman's Optineurin Monoclonal Antibody (Clone 8E12) can be used for immunocytochemistry applications.