Vimentin Monoclonal Antibody (Clone 12E4)

Vimentin is a cytoskeleton intermediate filament protein.{42183} It is composed of monomers that each contain a central ?-helix rod domain, which facilitates formation of a coiled-coil dimer required for vimentin filament assembly, as well as N-terminal head and C-terminal tail domains.{42183,42184} It is expressed in mesenchymal stem cells and cells of mesenchymal origin, including leukocytes, endothelial cells, and smooth muscle cells.{60331} Vimentin is attached to nuclei, endoplasmic reticulum, and mitochondria, and has a role in positioning organelles in the cytosol.{42184} It regulates glial morphology, facilitates motility and directional migration of fibroblasts, and is critical to mechanotransduction of shear stress and maintenance of vascular endothelial integrity.{42183} Vimentin controls transport of LDL-derived cholesterol from lysosomes to esterification sites.{42185} It is an aggresome component, forming a cage-like structure around aggregated, undegraded proteins at the microtubule organizing center.{42186} Vimentin is subject to citrullination under high calcium concentrations, which can occur during macrophage apoptosis, and citrullinated vimentin has been shown to have a role in the production of anti-citrullinated protein antibodies (ACPAs).{31883,31884} ACPAs against citrullinated proteins, such as vimentin, are considered to be highly specific markers for rheumatoid arthritis and other autoimmune diseases.{31883} Cayman's Vimentin Monoclonal Antibody (Clone 12E4) can be used for ELISA, immunoprecipitation (IP), and Western blot (WB) applications. The antibody recognizes vimentin at approximately 54 kDa from human samples.