Neuropeptide Y is a peptide belonging to the PP-family and occurs in neurons and adrenal medullary cells. Antisera raised against NPY often cross-react with PP and PYY. This antiserum was raised using a synthetic peptide from the prepro-NPY sequence which has no homologies to PP and NPY. Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining.
Positive control: Stefanini-fixed sections of rat small intestine.