Neuromedin U was found in porcine spinal cord. Neuromedin U-8 is contained within a larger polypeptide form neuromedin U-25. Neuromedin U is present in central and peripheral neurons, particularly in the enteric nervous system. Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining.
Positive control: Frozen sections of chicken small intestine.