HHATL Polyclonal Antibody

Hedgehog acyltransferase-like (HHATL) is the mammalian homolog of yeast Gup1 that is encoded by the HHATL gene in humans.{53526} HHATL is a membrane-bound O-acyltransferase (MBOAT) superfamily member and contains multiple transmembrane domains common to this family but lacks acyltransferase function due to a single amino acid substitution of histidine for leucine at position 447.{53526,27302} It is localized to the endoplasmic reticulum (ER) and is expressed in the heart, skeletal muscle, and brain.{53526,27300,53527} HHATL colocalizes with sonic hedgehog (Shh) and is a negative regulator of Shh N-terminal palmitoylation, a post-translational modification that is critical for Shh signaling in neural development and embryogenesis.{27300} Hhatl-/- neonatal mice fail to develop proper suckling ability, leading to malnutrition and death by postnatal day 14.{53528} HHATL expression is decreased in six nasopharyngeal carcinoma cell lines, as well as in tissue isolated from patients with nasopharyngeal carcinoma or skin squamous cell carcinoma.{53529,53530} Cayman's HHATL Polyclonal Antibody can be used for flow cytometry (FC), immunofluorescence (IF), and Western blot applications. The antibody recognizes HHATL at ~60 kDa from human samples.