G-418 (sulfate)

G-418 is an aminoglycoside antibiotic.{22696} It is active against T. thermophila (IC50 = 10 µM). It inhibits the 80S ribosome and protein elongation during translation in a cell-free assay when used at a concentration of 2 nM.{66212} G-418 is used in molecular biology as a selective agent in cell lines or plasmids containing the neomycin resistance gene, neoR.{66211} It increases survival in mouse models of Salmonella, P. vulgaris, K. pneumoniae, or E. coli when administered at doses ranging from 0.8 to 4 mg/kg.{66213}