Rat anti Human G-CSF antibody, clone BVD13-3A5 recognizes human granulocyte colony stimulating factor (G-CSF), also known as Pluripoietin. In addition Filgrastim and Lenograstim are used to describe recombinant human G-CSF preparations. Human G-CSF is a 207 amino acid, ~20 kDa O-linked glycoprotein belonging to the IL-6 superfamily. There are potentially three isoforms formed by alternative splicing, Rat anti Human G-CSF antibody, clone BVD13-3A5 binds the canonical isoform-1, binding to other isoforms has not been assessed (
UniProt: P09919).G-CSF is produced by activated macrophages, monocytes and neutrophils. In addition it can be produced by fibroblasts and endothelial cells (
Zsebo et al. 1988). G-CSF stimulates production of cells belonging to the granulocytic lineage in the bone marrow and is an activating agent for mature neutrophilic granulocytes (
Scapini et al. 2003) to produce other cytokines including CD257 (BAFF), operating through binding to the G-CSF receptor CD114.Rat anti Human G-CSF antibody, clone BVD13-3A5 has been successfully evaluated as a capture reagent in a highly sensitive sandwich ELISA assay with biotinylated Rat anti Human G-CSF, clone BVD11-37G10 (
1012808) as a detection reagent and recombinant human G-CSF (
PHP082B) as antigen.