WWL70

WWL70 is an inhibitor of ?/?-hydrolase domain-containing protein 6 (ABHD6; IC50 = 70 nM).{15297} It increases the expression of the adipose browning-related gene Ucp1 in differentiated 3T3-L1 mouse adipocytes and increases the oxygen consumption rate (OCR), an effect that can be blocked by the PPAR? antagonist GW 6471 (Item No. 11697), when used at a concentration of 10 µM.{53349} WWL70 (10 mg/kg per day) also increases the expression of the adipose browning-related genes Ucp1, Prdm16, Tmem26, and Tbx1 in visceral adipose tissue in mice fed a high-fat diet. WWL70 reduces adipose tissue mass and prevents glucose-intolerance and increases in body weight in mice fed a high-fat diet but does not reduce hepatic triacylglycerol levels.{53350} It increases brain levels of 2-arachidonoyl glycerol (2-AG; Item No. 62160) and decreases the severity of experimental autoimmune encephalomyelitis (EAE) in wild-type, but not cannabinoid (CB) receptor 2 (CB2) knockout, mice when administered at a dose of 10 mg/kg per day starting at disease onset.{53351}