S1P1 Blocking Peptide

To be used in conjunction with Cayman’s S1P1 Polyclonal Antibody (Item No. 10005228) to block protein-antibody complex formation during immunochemical analysis of S1P1. Sphingosine-1-phosphate (S1P) exerts its activity by binding to five distinct G-protein-coupled receptors, S1P1/EDG-1, S1P2/EDG-5, S1P3/EDG-3, S1P4/EDG-6, and S1P5/EDG-8.{11895,12477} S1P1 primarily couples with pertussis toxin-sensitive Gi/o proteins to mediate S1P-induced cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis.{11895,12477,10628} Expression of S1P1 is abundant in embryological vasculature and ubiquitous in adult cells, suggesting diverse physiological functions of this receptor.{12477} The human and murine S1P1 have 382 amino acids with an estimated molecular weight of 43 kDa. Glycosylation at the N-terminal extracellular domain may cause the protein to migrate at higher position in SDS-PAGE.{12476} Cayman’s S1P1 Polyclonal Antibody detects the receptor at 47 kDa in western blot. The antibody can also be used in immunostaining to study expression patterns of this protein.