S-Nitrosylated Protein Detection Kit (Biotin Switch)

Nitric oxide (NO) is produced by three distinct isoforms of nitric oxide synthase and functions as a key signaling molecule in physiology and pathophysiology.{9345,8644} NO transduces its effects by reacting either directly with heme and non-heme centers of proteins or indirectly via further oxidation to various reactive nitrogen species (RNS). Cayman’s S-Nitrosylated Protein Detection Kit (Biotin Switch) employs a modification of the Jaffrey et al. 'Biotin-switch' method to allow for the direct visualization of S-NO proteins in whole cells or tissues, as well as by western blot analysis.{12802,13441} Using this method, free SH groups are first blocked (an addition of 125.1 amu per residue) and any S-NO bonds present in the sample are then cleaved. Biotinylation of the newly formed SH groups (an addition of 523.6 amu per residue) provides the basis for visualization using streptavidin-based colorimetric or fluorescence detection.