Doppel Polyclonal Antibody

Doppel is a homolog of the cellular prion protein. Like prion protein, doppel has two N-linked oligosaccharides, and is presented on the cell surface via a glycosylphosphatidylinositol anchor.{11663} Unlike prion protein, it lacks the conformationally plastic and octapeptide repeat domains.{11604} The primary physiological role of doppel protein remains to be determined, but there is some evidence suggesting that cell surface prion protein can antagonize the toxic effect of doppel expressed in the central nervous system.{11663} In addition, the protein may play a major role in human male fertility, given its expression on both sertoli cells and spermatozoa.{11666} Doppel is differentially glycosylated, causing it to migrate at multiple sizes on SDS-PAGE.{11663} Cayman’s Doppel Polyclonal Antibody can be used for immunohistochemistry and Western blot applications. The antibody recognizes Doppel at 19 kDa (nonglycosylated) and 25 kDa (monoglycosylated) from human, mouse, and rat samples.