The adaptive immune response to SARS-CoV-2 includes specific antibody and T cell responses. The identification of a subpopulation of specific CD4 effector T cells that present the activation markers CD134 (OX40) and CD25 on their surface, which can be detected and quantified by flow cytometry after incubating blood with mitogens and/or specific antigens of pathogens to study, has demonstrated its utility in other infections

In this webinar, Eduardo López Granados, MD, PhD and Luz Yadira Bravo Gallego, MD explain their preliminary experience as an Immunology laboratory in the setting-up of the OX40 assay for the identification of CD4+ T cell specific responses to SARS-CoV-2 peptides, together with its ongoing application to determine the extent and characteristics of the response to natural infection and vaccination.

Capabilities of the OX40 assay to determine specific SARS-CoV-2 T cell responses