Ready-to-Use 3D Blood Brain Barrier Spheroids with Human BRAIN Microvascular Endothelial Cells
ScienCell offers cryopreserved 3D multicellular BBB spheroids comprised of human brain microvascular endothelial cells (HBMEC), brain vascular pericytes, and astrocytes at a 1:1:1 ratio.
Product description
The blood brain barrier (BBB) is a specialized capillary bed that separates the brain from the circulatory system and protects the brain from most pathogens. Endothelial tight junctions supported by pericytes and astrocytes are primarily responsible for the highly selective nature of the BBB, restricting the passage of numerous solutes, most antibodies, and some antibiotics. As such, efforts to understand the mechanisms underlying BBB integrity have been critical to developing techniques that are able to penetrate the BBB to deliver therapeutic or diagnostic molecules to the brain. Due to the complexities of the BBB, it is difficult to study in a 2-dimensional in vitro system, which inherently lacks multiple aspects of the physiological microenvironment. ScienCell™’s ready-to-use 3D human blood brain barrier spheroid kit offers cryopreserved 3D multicellular BBB spheroids comprised of human brain microvascular endothelial cells (HBMEC), brain vascular pericytes, and astrocytes at a 1:1:1 ratio, recapitulating the intracellular interactions at BBB. A highly unique feature of this kit is that researchers can achieve the functional and homogenous 3D BBB spheroids in 24-48 hours after thawing (Fig. 1-3), without encountering the long and complex procedures involved in 3D cell culture.
