One of the earliest indications of apoptosis is the translocation of phosphatidylserine from the inner to the outer leaflet of the plasma membrane. Once exposed to the extracellular environment, phosphatidylserine becomes available for annexin V, a 35–36 kDa Ca2+ dependent phospholipid-binding protein with a high affinity for it. The early apoptotic cells have still intact plasma membrane, so their DNA is not accessible for propidium iodide dye, and these cells are annexin V positive and propidium iodide negative, unlike viable cells (annexin V negative, propidium iodide negative) and necrotic cells or late apoptotic cells (annexin V positive, propidium iodide positive).
EXBIO offers a complex solution for apoptosis detection via flow cytometry method: ApoFlowEx kit, annexin V in multiple formats, annexin V binding buffer and antibodies to apoptosis related antigens.
Analysis of camptothecin treated (A, apoptosis induced) and not treated cells (B) upon staining with ApoFlowEx FITC kit.
Annexin V – purified and fluorescently labelled
|EXB0023||Dyomics 647||FC||100 tests|
|EXB0030||Dyomics 647||FC||500 tests|
|EXB0035 (coming soon)||Purified||FC||0,1 mg|
|EXB0031 (coming soon)||Biotin||FC||0,1 mg|
Flow cytometry staining of apoptotic Jurkat cells with 7-AAD and annexin V FITC.
CellCycleFlowEx is intended for cell cycle analyses based on measuring of DNA content using flow cytometry. It enables to recognize the cells in G0/G1 phase (the same amount of DA as the resting cells), S phase (in the process of DNA duplication), and G2/M phase (having double the amount of DNA as the resting cells). The kit is suitable for testing suspensions of isolated cells, such as leukocytes isolated from peripheral blood (PBMC) or cells from tissue culture.
Analysis of A) PBMC stimulated with PHA (5 ug/ml) for 3 days and B) unstimulated cells.
Distribution of cells in the singlet gate according to the DNA content into G0/G1, S and G2/M phases of the cell cycle.