StarBright Dyes are unique, fluorescent nanoparticles conjugated to Bio-Rad’s highly validated antibodies. Developed specifically for flow cytometry to give you exceptional brightness with exacting excitation and emission.
The brightness allows easy resolution of rare populations and low density antigens while maintaining the flexibility to fit into any multicolor panel.
See how the features of StarBright Dyes can enhance your flow cytometry experiment.
- Narrow excitation and emission
- Flexible as no special buffers required
- Compatible with all instruments and reagents
- Lot-to-lot reproducibility
StarBright Dyes have been designed to have superior brightness with defined excitation and emission characteristics. This allows better resolution of specific cell populations, including rare and low density antigens, while minimizing spillover and spreading in multicolor panels. With StarBright Dyes you can build bigger panels with ease.
StarBright Dyes easily fit into your existing protocols and panels. They are compatible with most staining protocols, work in virtually any buffers, including commercial polymer staining buffers, with no loss in performance (Figure 2). They can be combined with other StarBright Dyes, organic fluorophores, protein-based fluorophores, and other polymer dyes without the need for special staining protocols (Figure 2C), allowing you to build larger panels with ease.
StarBright Dyes have been developed on the ZE5 Cell Analyzer but can be used on any instrument with the right lasers and filters, including cell sorters. In addition, they have been shown to work in spectral flow cytometry giving comparable results to conventional flow.
StarBright Dyes, unlike some polymer and other dyes, are not tandem dyes. This means they can be made with high lot-to-lot reproducibility (Figure 4), are stable and resistant to photobleaching with no loss of performance over time. They also exhibit less cross-laser excitation and no excitation from acceptor dyes found in tandem dyes. Additionally, they can be fixed in paraformaldehyde with little or no drop in fluorescence.