Sanbio would like to highlight two of their suppliers in relation to 3D Cell Culture. High-quality biotechnology companies MBL International and ScienCell Research Laboratories.

First we explain more about Afamin/Wnt3a CM of MBL International. Secondly you will find an overview of the 3D Cell Culture products of ScienCell.

MBL International: Afamin/Wnt3a CM

Organoid Culture

Wnt signaling is known to be involved in early development, maintenance and regeneration of stem cells, and in cancer formation. Wnt signaling has also been found to play an important role in the growth and maintenance of these processes. In particular, Wnt3a has been revealed to be an essential niche component for maintaining the proliferation of Lgr5-positive stem cells in intestinal epithelial cells and is used for the production of various digestive organoids such as the small intestine, large intestine, stomach, pancreas and liver. Although Wnt3a has been conventionally used for the culture of gut organoids, it is a fat-soluble protein, so it forms aggregates in serum-free medium and cannot exert its activity sufficiently. In 2016, Mihara et al. found that high Wnt3a activity can be maintained by forming a complex with Wnt3a by Afamin, which is one of the components of serum. In addition, by using Afamin and Wnt3a complex for organoid culture, long-term culture of organoid becomes possible. This new medium will result in optimal success for your organoid experiments.

 Mechanism of Wnt3a Stabilization by Afamin Proteins

Afamin/Wnt3a CM increased Lgr5 Positive Stem Cells Expressed td Tomato

ScienCell: 3D Cell Culture portfolio

The California-based biotechnology company ScienCell is the largest supplier of normal primary cells. Within their large portfolio of products they also offer 3D Cell Culture products.

Key features of  ScienCell's 3D Cell Culture Products

• Convenient: Ready-To-Use 3D spheroids are delivered frozen.
• Comprehensive: All-Inclusive 3D Cell Culture Kits contain everything you need for performing 3D cell culture.
• Easy-to-use: No prior experience in 3D cell culture is required.
• Functional: Cell-specific functional markers are maintained in 3D cell culture.
• Reproducible: 3D cell structures form in a robust and homogeneous manner.

3D Ready-To-Use Cell Culture Spheroids Kits

Unique features

• Innovative: ScienCell is the only company to offer Ready-To-Use spheroids.
• Ready-to-use: Obtains hundreds of uniform 3D spheroids per well in 24 hours after thawing.
• High-throughput: Just thaw and plate spheroids directly into 24-, 48-, or 96-well plates.
• Simple: No experience in 3D cell culture is required.
• Functional: Maintains the expression of functional markers.

Gain New Biological Insights with ScienCell’s Ready-to-use 3D Spheroids

An overview of the Ready-to-use 3D Cell Culture Spheroid Kits

All-Inclusive 3D Cell Culture Spheroid Kits

Key features

• Convenient: Easy-to-use comprehensive kits.
• Flexible: Allows manipulations of specific variables using the All-Inclusive 3D Cell Culture Spheroid Kits.
• Uniform: Hundreds of 3D spheroids develop uniformly in each well using specially-formulated 3D cell culture medium.
• Reproducible: Obtains a statistically significant sample size from hundreds of 3D spheroids per well, enhancing the accuracy of your experimental data.

Homogenous Formation of 3D Primary Spheroids

An overview of the All-Inclusive 3D Cell Culture Spheroid Kits

Collagen I-Based 3D Cell Culture Kits

Main Features

• Convenient: Easy-to-use comprehensive kits.
• Relevant: Utilizes extracellular matrix to mimic the in vivo
• Reproducible: Achieves reliable 3D experimental results using highly quality primary cells and reagents from ScienCell.
• Functional: Displays cell-specific functional markers in ScienCell’s 3D collagen-based models.

3D Blood Brain Barrier (BBB) Model

Overview of the Collagen-Based 3D Cell Culture Kits

3D Cell Culture Related Products

To complement the wide-range of 3D cell culture kits, ScienCell also offers 3D cell culture related products. ScienCell strives to provide you with reliable cell culture products and technical support to help you ease transition from 2D to 3D culture models.