Sanbio would like to highlight two of their suppliers in relation to 3D Cell Culture. High-quality biotechnology companies MBL International and ScienCell Research Laboratories.

First we explain more about Afamin/Wnt3a CM of MBL International. Secondly you will find an overview of the 3D Cell Culture products of ScienCell.

MBL International: Afamin/Wnt3a CM

Organoid Culture

Wnt signaling is known to be involved in early development, maintenance and regeneration of stem cells, and in cancer formation. Wnt signaling has also been found to play an important role in the growth and maintenance of these processes. In particular, Wnt3a has been revealed to be an essential niche component for maintaining the proliferation of Lgr5-positive stem cells in intestinal epithelial cells and is used for the production of various digestive organoids such as the small intestine, large intestine, stomach, pancreas and liver. Although Wnt3a has been conventionally used for the culture of gut organoids, it is a fat-soluble protein, so it forms aggregates in serum-free medium and cannot exert its activity sufficiently. In 2016, Mihara et al. found that high Wnt3a activity can be maintained by forming a complex with Wnt3a by Afamin, which is one of the components of serum. In addition, by using Afamin and Wnt3a complex for organoid culture, long-term culture of organoid becomes possible. This new medium will result in optimal success for your organoid experiments.

Article number Description
J-ORMW301R Afamin/Wnt3a CM

 Mechanism of Wnt3a Stabilization by Afamin Proteins

Afamin/Wnt3a

Afamin/Wnt3a CM increased Lgr5 Positive Stem Cells Expressed td Tomato

MBL Afamin-Wnt3a

ScienCell: 3D Cell Culture portfolio

The California-based biotechnology company ScienCell is the largest supplier of normal primary cells. Within their large portfolio of products they also offer 3D Cell Culture products.

Key features of  ScienCell's 3D Cell Culture Products

  • Convenient: Ready-To-Use 3D spheroids are delivered frozen.
  • Comprehensive: All-Inclusive 3D Cell Culture Kits contain everything you need for performing 3D cell culture.
  • Easy-to-use: No prior experience in 3D cell culture is required.
  • Functional: Cell-specific functional markers are maintained in 3D cell culture.
  • Reproducible: 3D cell structures form in a robust and homogeneous manner.

3D Ready-To-Use Cell Culture Spheroids Kits

Unique features

  • Innovative: ScienCell is the only company to offer Ready-To-Use spheroids.
  • Ready-to-use: Obtains hundreds of uniform 3D spheroids per well in 24 hours after thawing.
  • High-throughput: Just thaw and plate spheroids directly into 24-, 48-, or 96-well plates.
  • Simple: No experience in 3D cell culture is required.
  • Functional: Maintains the expression of functional markers.

Gain New Biological Insights with ScienCell’s Ready-to-use 3D Spheroids

Ready-to-use 3D Cell Culture Spheroids

An overview of the Ready-to-use 3D Cell Culture Spheroid Kits

3D Cell Culture Spheroid Kits

Article number Label in above image Description
SCCSP3D-8768 Blood Brain Barrier Model Ready-To-Use 3D Human Blood Brain Barrier Spheroid Kit with HBMEC
SCCSP3D-8738 Blood Brain Barrier Model Ready-to-use 3D Human Blood Brain Barrier Spheroid Kit
SCCSP3D-5000 Liver Model Ready-to-use 3D Hepatic Stellate-Endothelial Cell Spheroids
SCCSP3D-5300 Liver Model Ready-to-use 3D Hepatocyte-Stellate Cell Spheroids
SCCSP3D-8750 Liver Model Ready-to-use 3D Human Hepatic Stellate Cell Monoculture Spheroids
SCCSP3D-1520 Cerebral Cortex Model Ready-to-use 3D Human Cortical Spheroids
SCCSP3D-6540 Ocular Model Ready-To-Use 3D Human Retinal Pigment Epithelial Spheroids
SCCSP3D-3200 Lung Model Ready-to-use 3D Human Pulmonary Alveolar Epithelial Cell Spheroids
SCCSP3D-3210 Lung Model Ready-To-Use 3D Human Airway Spheroids
SP3D-4100 Kidney Model Contact support@sanbio.nl for more information.
SP3D-4108 Kidney Model Contact support@sanbio.nl for more information.
SP3D-7500 Mesenchymal Stem Cell Model Contact support@sanbio.nl for more information.
SCCSP3D-4650 Cartilage Model Ready-to-use 3D Human Chondrocyte-articular Spheroid Kit
SCCSP3D-8748 Bone Model Ready-to-use 3D Osteogenesis-Angiogenesis Coupling Kit
SCCSP3D-4600 Bone Model Ready-to-use 3D Human Calvarial Osteoblast Spheroid Kit

All-Inclusive 3D Cell Culture Spheroid Kits

Key features

  • Convenient: Easy-to-use comprehensive kits.
  • Flexible: Allows manipulations of specific variables using the All-Inclusive 3D Cell Culture Spheroid Kits.
  • Uniform: Hundreds of 3D spheroids develop uniformly in each well using specially-formulated 3D cell culture medium.
  • Reproducible: Obtains a statistically significant sample size from hundreds of 3D spheroids per well, enhancing the accuracy of your experimental data.

Homogenous Formation of 3D Primary Spheroids

3D Cell Culture Primary Spheroids

An overview of the All-Inclusive 3D Cell Culture Spheroid Kits

All-Inclusive 3D Cell Culture Spheroid Kits

Article number Label in above image Article description
SCC3D-8738 Blood Brain Barrier Model All-inclusive 3D Human Blood Brain Barrier Spheroid Formation Kit
SCC3D-R8738 Blood Brain Barrier Model All-inclusive 3D Rat Blood Brain Barrier Spheroid Formation Kit
SCC3D-5000 Liver Model All-Inclusive 3D Human Hepatic Stellate-Endothelial Cell Spheroid Formation Kit
SCC3D-8750 Liver Model All-inclusive 3D Human Hepatic Stellate Cell Monoculture Spheroid Formation Kit
SCC3D-1520 Cerebral Cortex Model All-Inclusive 3D Human Cortical Spheroid Formation Kit
SCC3D-6540 Ocular Model All-inclusive 3D Human Retinal Pigment Epithelial Spheroid Formation Kit
SCC3D-4650 Cartilage Model All-inclusive 3D Human Chondrocyte-articular Spheroid Formation Kit
SCC3D-8748 Bone Model All-inclusive 3D Osteogenesis-Angiogenesis Coupling Kit
SCC3D-4600 Bone Model All-inclusive 3D Human Calvarial Osteoblast Spheroid Formation Kit

Collagen I-Based 3D Cell Culture Kits

Main Features

  • Convenient: Easy-to-use comprehensive kits.
  • Relevant: Utilizes extracellular matrix to mimic the in vivo
  • Reproducible: Achieves reliable 3D experimental results using highly quality primary cells and reagents from ScienCell.
  • Functional: Displays cell-specific functional markers in ScienCell’s 3D collagen-based models.

3D Blood Brain Barrier (BBB) Model

3D Blood Brain Barrier Model

Overview of the Collagen-Based 3D Cell Culture Kits

Collagen-Based 3D Cell Culture Kits

Article number Label in above image Article description
SCC8738 Blood Brain Barrier Model Human Blood Brain Barrier Modeling Kit
SCC8728 Blood Brain Barrier Model 3D Endothelial–Pericyte Coculturing Kit
Kidney Model
SCC8708 Vascular Biology Model 3D Embedded Tubule Formation Kit
SCC8718 Vascular Biology Model 3D Network Formation Assay Kit

3D Cell Culture Related Products

To complement the wide-range of 3D cell culture kits, ScienCell also offers 3D cell culture related products. ScienCell strives to provide you with reliable cell culture products and technical support to help you ease transition from 2D to 3D culture models.

Article number Article description
SCC0353 Ultra-Low Binding Culture Plate (96-well)
SCC0343 Ultra-Low Binding Culture Plate (48-well)
SCC0383 Ultra-Low Binding Culture Plate (24-well)
SCC0373 Ultra-Low Binding Culture Plate (6-well)
SCC8688 3D Collagen I Matrix Kit
SCC8178 Collagen I-3D Gelling Kit
SCC8808 3D Gel Staining Prep Kit
SCC8158 In Vitro Tube Formation Assay
SCC8698 3D Basic Embedded Tubule Formation Kit