BODIPY-aminoacetaldehyde diethyl acetal

BODIPY-aminoacetaldehyde diethyl acetal (BAAA-DA) is a stable precursor to BODIPY-aminoacetaldehyde, a cell-permeable fluorescent substrate for aldehyde dehydrogenase (ALDH).{28080,28081} BODIPY-aminoacetaldehyde diethyl acetal is converted under acidic conditions to BODIPY-aminoacetaldehyde (BAAA).{28081} BAAA is cell-permeant and is converted intracellularly by ALDH to BODIPY aminoacetate (BAA), which is retained by cells and can be used to identify cells with high ALDH activity.{28080} BAA is a substrate for the efflux pump P-glycoprotein (P-gp) but co-application of BAAA with a P-gp inhibitor, such as verapamil (Item No. 14288), inhibits BAA efflux.{28081} BAAA-DA has been used to isolate human hematopoietic progenitor cells, which have high ALDH activity, and via flow cytometry to sort cancer stem cells that contain high levels of ALDH.{28080,49664} BAA used in cells can be excited at 488 nm and displays an emission maximum of 512 nm.{49665}