(R)-nitro-Blebbistatin

(R)-nitro-Blebbistatin is a more stable form of (+)-blebbistatin (Item No. 13165), which is the inactive form of (-)-blebbistatin (Item No. 13013). Prolonged exposure to blue light (450-490 nm) results in degradation of blebbistatin to an inactive product via cytotoxic intermediates, which may be problematic for its use in fluorescent live cell imaging applications.{17038,24037} The addition of a nitro group stabilizes the molecule to circumvent its degradation by prolonged blue light exposure.{16725} (R)-nitro-Blebbistatin has the same stereochemistry as the inactive (+)-blebbistatin enantiomer.