Cayman’s Caspase-4 Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human caspase-4, a major upstream effector of non-canonical inflammasome activation. The assay uses a caspase-4-specific fluorogenic substrate, Ac-LEVD-AFC. Caspase-4 cleaves this substrate generating free AFC, which can be easily quantified using a fluorescence plate reader at excitation and emission wavelengths of 400 and 505 nm, respectively. The potent and reversible caspase-4 inhibitor AC-LEVD-CHO is included as a positive control.
Specifications
Size
384 Well
Supplier
Cayman Chemical
Shipping & storage
Shipping condition
Dry Ice
Storage temperature
-80°C