The mab reacts specifically with symplekin (a 150 kDa polypeptide) present in 1) tight junctions (zonula occludens) of polar epithelia and Sertoli cells of testis (but not their vascular endothelia!) and 2) exclusively in the nucleoplasm of these and many other cell types which do not form tight junctions (e.g. stratified epithelia such as oesophagus, tongue mucosa, epidermis; squamous cell carcinoma; non-epithelia such as fibroblasts, endothelia, lymphocytes). Polypeptide reacting: Symplekin, 126.5 kD polypeptide (calculated from aa sequence data); apparent Mr 150 kD (after SDS-PAGE); pI 6.5. Reactivities on Cultured Cell lines: Carcinoma cell lines (e.g. MCF-7, Caco-2, HT29, PLC), SV80 (fibroblasts), U333 CG / 343 MG (glioma), K 562 (erythroleukemia), RVF (rat vascular smooth muscle cells).
Specifications
Host
Mouse
Clonality
Monoclonal
Clone
Sym-TJ-E150
Supplier
PROGEN
Shipping & storage
Shipping condition
Room Temperature
Storage temperature
2-8°C

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