(+-)8(9)-EET

(+-)8(9)-EET is a fully racemic version of the R/S enantiomeric forms biosynthesized from arachidonic acid (Item No. 90010).{463,729} (+-)8(9)-EET is a major cytochrome P450 (CYP450) metabolite in the renal cortex.{1116} It activates PPARa in HEK293 cells when used at a concentration of 1 µM but inhibits NF-kB activity induced by IL-1B in a PPARa-dependent and -independent fashion.{42155} It is also a substrate of COX-1 and COX-2, as measured by oxygen consumption and product formation assays when used at a concentration of 50 µM.{34481} (8S,9R)-EET (1 µg/kg), but not (8R,9S)-EET, reduces the glomerular filtration rate (GFR) through COX-dependent pre-glomerular vasoconstriction in rats.{1111}