15-hydroxy Prostaglandin dehydrogenase (PGDH) is an enzyme of the short-chain alcohol dehydrogenase family that catalyzes the oxidation of prostaglandins to 15-keto metabolites with reduced biological activity.{8277,5201,340,55499} It uses NAD+ as a cofactor and, in addition to PGs, also converts various hydroxy fatty acids, such as HETEs, resolvins, and lipoxins, to less active keto metabolites.{55499,6339,15718,55500,55501} 15-PGDH acts as a tumor suppressor, and restoring HPGD, the gene encoding 15-PGDH, expression in human colon cancer cells in vitro reduces subsequent tumor formation in mouse xenograft models.{14568,14566,12480,18885} Knockout of Hpgd or inhibition of 15-PGDH in mice increases hepatic tissue regeneration and induces resistance to ulcerative colitis.{29053} Inhibition of 15-PGDH also increases mitochondrial function and autophagic flux in muscle tissue, as well as increases muscle mass and strength in aged mice.{59739} Cayman’s 15-PGDH D50H variant (human, recombinant) protein contains an aspartate-to-histidine substitution at position 50 (D50H) and can be used for enzyme assay and Western blot (WB) applications.