IgG Fab (rabbit) Monoclonal Antibody - Biotinylated

Immunoglobulin G (IgG) is a member of the immunoglobulin superfamily of glycoproteins that plays a central role in the adaptive immune response.{53880,53882} It is produced by B cells and later secreted by plasma cells and is the most abundant circulating antibody in rabbit serum. IgG consists of two identical heavy chains of approximately 50 kDa each and two identical light chains of approximately 25 kDa each.{53880} The heavy chains are linked together by a single disulfide bond to form an Fc region and also combine with the light chains through additional disulfide bonds to form the Fab region, which mediate receptor and antigen binding, respectively. IgG is produced following IgM class-switching in response to infection and is involved in numerous humoral host defense responses, including antibody-dependent cell-mediated cytotoxicity (ADCC), toxin neutralization, and pathogen opsonization.{55170,53880,53883} Rabbits express three light chains, IgGk (K1), IgGk (K2), and IgGy, with IgGk (K1)-containing IgGs comprising approximately 90% of the total IgG population. Unlike human and mouse IgG, rabbit IgG has a single subclass and a short upper and middle hinge length of only 11 residues that facilitates the hinge extension necessary for binding of the Fc receptor (FcR) and activating complement component 1q (C1q) to facilitate complement activation.{53882,53880} Cayman's IgG (rabbit) Monoclonal Antibody - Biotinylated can be used for ELISA and Western blot (WB; non-reducing conditions) applications. The antibody recognizes the Fab region of IgG at approximately 150 kDa from rabbit samples.